The provided Anaerobic Culture Jar is utilized to make an oxygen-safe environment for the development of anaerobic microorganisms. It is integrated with inoculated tubes or plates in the chamber. The anaerobic conditions are made by including a liquid to a gas producer envelope, which is positioned in the jar only prior to sealing. The envelope has dual chemical tablets such as sodium bicarbonate and sodium borohydride. Water responds with these chemicals inside our Anaerobic Culture Jar, generating carbon dioxide and hydrogen gas. In addition, the hydrogen gas blends with free gas in the container to generate water by expelling every free oxygen.